Leukocytes
Isolation of Leukocytes with Collagenase NB
In the following, application details for isolation of Leukocytes from murine skin are presented.
Species | Enzyme | Conditions | Download |
Murine* | Collagenase NB 7D | 0.18-0.22 PZ U/ml, | Protocol |
* For information on GMP grade Collagenase NB please contact us!
Collagenase NB 7D Gentle Grade (Cat. No. S1747402) is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
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