Isolation of Leukocytes with Collagenase NB

In the following, application details for isolation of Leukocytes from murine skin are presented.

Species Enzyme ConditionsDownload
 Murine*Collagenase NB 7D

0.18-0.22 PZ U/ml,
30 min, 37 °C


 * For information on GMP grade Collagenase NB please contact us!

Collagenase NB 7D Gentle Grade (Cat. No. S1747402)  is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Isolation conditions

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.

For further information on optimization of tissue digestion please visit our support section!

Contact Nordmark Biochemicals Team 

Product Inquiry

Product Inquiry

Product Inquiry Sidebar

Personal Consulting


+49 (0)4122 / 712 560