Acinar Cells

Isolation of acinar cells with Collagenase NB

In the following, application details for isolation of acinar cells from human pancreatic tissue are presented.

Species Enzyme  Conditions  Download
 Human*  Collagenase NB 8 0.05-0.1 PZ U/ml, 
1 h, 37 °C
Protocol

 * For information on GMP grade Collagenase NB please contact us!

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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